D1.1: At least four target dyads designed on the paper (together with the respective monomers).
SUCCESSFULLY COMPLETED.
D1.2: At least one monomer synthesized in ca. 30 mg quantity and at least one of the dyads synthesized in ca. 10 mg quantity.
SUCCESSFULLY COMPLETED.
D2.1: Three target dyads designed on the paper.
SUCCESSFULLY COMPLETED.
D2.2: At least one target dyad synthesized in ca. 20 mg quantity.
SUCCESSFULLY COMPLETED.
D3.1: At least one dyad will perform sufficiently well to be delivered for microscopy validation.
SUCCESSFULLY COMPLETED.
D4.1: A log-log plot of fluorescence intensity versus excitation intensity (slope = 4 for a quartic dependence).
SUCCESSFULLY COMPLETED.
D4.2: Determination of the optimal Performance (0-1) for each of the validated compounds.
SUCCESSFULLY COMPLETED.
D5.1: At least one dyad that has been equipped with a solubilizing group.
ONGOING
D5.2: At least one dyad that passes all characterization/validation criteria.
D6.1: At least one dyad conjugated to a secondary antibody or peptide.
D6.2: A log-log plot to see if the quartic dependence is achievable in the cells and recommendations as for optimal Performance values in the cells.
D7.1: Signed Consortium Agreement.
COMPLETED.
D7.2: Opening and maintaining the website and social media channels.
ONGOING.
D7.3: Organization of presential meetings.
ONGOING.
D7.4: First press release.
ONGOING.
D7.5: Data Management Plan.
ONGOING.
D7.6: A first version of the Dissemination and Exploitation Plan.
ONGOING.
D7.7: End User Panel established.
COMPLETED.
D7.8: Midterm and final report.
D7.9: Final press release.
Publications
Chen-Wu J, Benitez-Martin C, González-Delgado JA, de Jong F, Fron E, Steurs G, Martínez-Martínez AJ, Nájera F, Grøtli M, Hofkens J, Andréasson J, Pischel U. Heteroaryl iminothioindoxyl (HA-ITI) photoswitches via regioselective aza-Wittig synthesis: unifying red-shifted absorption, large E/Z band separation, and tunable thermal recovery. Chem Sci. 2025 Dec 24. doi: 10.1039/d5sc08074f. Epub ahead of print.
Replication Data for: Heteroaryl Iminothioindoxyl (HA-ITI) Photoswitches via Regioselective Aza-Wittig Synthesis: Unifying Red-Shifted Absorption, Large E/Z Band Separation, and Tunable Thermal Recovery”, https://doi.org/10.48804/LXAEID, KU Leuven RDR, V1
Benitez-Martin C, Rouillon J, Fron E, de Jong F, Grøtli M, Hofkens J, Pischel U, Andréasson J. Exploiting negative photochromism to harness a four-photon-like fluorescence response with two-photon excitation. Nat Commun. 2025, 16, 10897. doi: 10.1038/s41467-025-66602-1.
Replication Data for ‘Exploiting negative photochromism to harness a four-photon-like fluorescence response with two-photon excitation’”, https://doi.org/10.48804/WHKQSH, KU Leuven RDR, V1