WP1: Design and synthesis of red-shifted photoswitches and the corresponding dyads (1st generation)

WP2: Design and synthesis of additional dyads (1st generation)

WP3: Spectroscopic characterization of the compounds synthesized in WP1 and WP2

WP4: Validate the synthesized and spectroscopically characterized dyads in polymer matrices or small sample volumes using two-photon microscopy.

WP5: Design, synthesis, and characterization/validation of water-soluble probes (2nd generation)

WP6: Bioconjugation to secondary antibodies or cell-penetrating peptides for cell studies using multiphoton microscopy.

WP7: Coordination of research, financial management, reporting to EC, and dissemination